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KMID : 0363620180390010001
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Journal of Korean Oriental Medicine
2018 Volume.39 No. 1 p.1 ~ p.12
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Effects of Topical application of Astragalus membranaceus in Spontaneous Alopecia Mice Model
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Kwon Hyeok-Je
Kim Mi-Hye
Yang Woong-Mo
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Abstract
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Objectives: Astragalus membranaceus has been reported to inhibit immune responses, but its effect on hair loss is not clear. In this study, the effect of A. membranaceus extract (AM) on hair regrowth in C57BL/6 mice with natural hair loss in the telogen phase was investigated.
Methods: Mice with natural hair loss were topically treated with 1% AM on the dorsal skin for 2 weeks. Dorsal skin samples were stained with hematoxylin and eosin and probed with an anti-mouse CD8a IgG. The mRNA expression levels of tumor necrosis factor (TNF)-α, interferon (IFN)-γ and interleukin (IL)-4 were measured by reverse transcription polymerase chain reaction and quantitative real-time polymerase chain reaction.
Results: AM treatment induced hair regrowth in hair loss mice, while control mice suffered continued hair loss. Tapering hair shafts and broken hair follicles were decreased as well as CD8+ T lymphocyte infiltration. In addition, the expressions of TNF-α, IFN-γ and IL-4 were reduced by AM treatment. Also, AM treatment significantly increased the KGF expressions in Hs68 fibroblast cells.
Conclusion: These results suggest that topical application of A. membranaceus may be an alternative therapy for hair loss.
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KEYWORD
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Astragalus membranaceus, hair loss, hair follicle, T lymphocyte, cytokine
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